Extended Data Fig. 6: Cryo-EM analysis of the PspCas13b–crRNA–tgRNA ternary complex.
From: Structural insights into RNA-guided RNA editing by the Cas13b–ADAR2 complex
(a) Single-particle cryo-EM image processing workflow. (b) Representative micrograph at a magnification of ×105,000. (c) Representative 2D averaged class images. (d and e) Cryo-EM density maps of State 1 (d) and State 2 (e), colored according to the local resolution. (f and i) FSC curves of State 1 (f) and State 2 (i). The map-to-map FSC curves were calculated between the two independently refined half-maps after masking (blue line), and the overall resolutions were determined by the gold standard FSC = 0.143 criterion. The map-to-model FSC curves were calculated between the refined atomic models and maps (red line). (g and j) Directional FSC plots of State 1 (g) and State 2 (j), calculated by the 3DFSC server. (h and k) Angular distributions of the particles in the final reconstructions of State 1 (h) and State 2 (k). (l and m) Cryo-EM density maps of State 1 (l) and State 2 (m), colored according to the structural domains.
