Extended Data Fig. 7: Structural comparison of the PspCas13b binary and ternary complexes.
From: Structural insights into RNA-guided RNA editing by the Cas13b–ADAR2 complex
(a) Surface representations of the PspCas13b binary complex (left) and ternary complexes in State 1 (middle) and State 2 (right). The active sites are indicated by dashed red lines. (b) Recognition of the guide–target duplex in the PspCas13b ternary complexes in States 1 (left) and State 2 (right). (c) Superimposition of the PspCas13b State 2 ternary complex (colored as in a) and the HEPN domains of the binary complex (colored blue), based on their HEPN1 domains. A potential steric clash between the State 2 ternary complex and the binary complex is indicated by a dashed orange circle. (d–f) Lid loop structures in the binary complex (d) and the ternary complexes in State 1 (e) and State 2 (f).
