Extended Data Fig. 6: NsEM epitope mapping of the RUPA-71 binding site on Pfs48/45 D1D2 (a) and comparison of the RUPA-58 and putative RUPA-71 binding sites on D1D2 (b). | Nature Structural & Molecular Biology

Extended Data Fig. 6: NsEM epitope mapping of the RUPA-71 binding site on Pfs48/45 D1D2 (a) and comparison of the RUPA-58 and putative RUPA-71 binding sites on D1D2 (b).

From: Structural elucidation of full-length Pfs48/45 in complex with potent monoclonal antibodies isolated from a naturally exposed individual

Extended Data Fig. 6

(a) 2D classes and a 3D reconstruction of the RUPA-154:RUPA-71:Pfs48/45-D1D2 complex generated using nsEM. A predicted structure of RUPA-71 generated from Abodybuilder2 and the RUPA-154-D1D2 portion of the full-length Pfs48/45-Fab cryoEM structure were used to model this complex. RUPA-71 (dark violet), RUPA-154 (yellow), and D1D2 (blue) are depicted as ribbons within the nsEM map. The putative epitope of RUPA-71 is shown in coral. (b) RUPA-58 (light gray) and RUPA-71 (dark violet) are depicted with ribbons and surface bound to Pfs48/45-D1D2 (blue) which is represented as ribbons.

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