Extended Data Fig. 8: Mutation N131Q reduces binding of potent Pfs48/45 domain 1 antibodies. | Nature Structural & Molecular Biology

Extended Data Fig. 8: Mutation N131Q reduces binding of potent Pfs48/45 domain 1 antibodies.

From: Structural elucidation of full-length Pfs48/45 in complex with potent monoclonal antibodies isolated from a naturally exposed individual

Extended Data Fig. 8

BLI data of RUPA-71 (a), RUPA-116 (b), RUPA-119 (c), and RUPA-75 (d), RUPA-155 (e), and RUPA-46 (f) binding to wild-type Pfs48/45-D1D2 and a D1D2 construct containing a N131Q point mutation. This mutation removes a putative N-linked glycosylation sequon. RUPA-58 was used as a control for proper folding of the D1D2 N131Q mutant (g). (h) A model of RUPA-71 (dark violet) binding to D1D2 (blue) generated using nsEM with a glycan at position N131 modeled in coral, which would result from expression of the D1D2 construct in mammalian cells. Source data for panels a-g are available online.

Source data

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