Extended Data Fig. 3: CryoEM analysis of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 and Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complexes. | Nature Structural & Molecular Biology

Extended Data Fig. 3: CryoEM analysis of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 and Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complexes.

From: Structural elucidation of full-length Pfs48/45 in complex with potent monoclonal antibodies isolated from a naturally exposed individual

Extended Data Fig. 3

(a) Representative cryoEM micrograph (from 1529 collected) of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex. (b) Selected 2D class averages of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex. (c) Gold standard Fourier shell correlation (GSFSC) curves for the final 3D non-uniform refinement of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex in cryoSPARC v3. (d) Viewing direction distribution of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex dataset. (e) Local resolution (Å) plotted on the surface (left panels) and center (right) of the cryoEM map of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex. (f) Representative cryoEM micrograph (from 3435 collected) of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex acquired with no tilt applied. (g) Selected 2D class averages of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex. (h) GSFSC curves for the final 3D non-uniform refinement of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 in cryoSPARC v4. (i) Viewing direction distribution of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex dataset. (j) Local resolution (Å) plotted on the surface (top panels) and center (bottom) of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex cryoEM map. (k) Representative cryoEM micrograph (from 2785 collected) of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex collected under 40o tilt. (l) Selected 2D class averages of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex. (m) GSFSC curves for the final 3D non-uniform refinement (top) and the final 3D flexible refinement (bottom) of the Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex in cryoSPARC v4. (n) Viewing direction distribution of the Pfs48/45-scTB31F:RUPA-44:RUPA-58 complex dataset. (o) Local resolution (Å) plotted on the surface of the flexibly refined Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex cryoEM map (top panels) and on the surface (bottom left panels) and center (bottom right panel) of flexibly refined Pfs48/45-scTB31F:RUPA-44:RUPA-58:RUPA-154 complex cryoEM map after processing with EMReady2. (p) GSFSC curves for the final locally refined map of the D1D2:RUPA-58:RUPA-154 region in cryoSPARC v4. (q) Local resolution (Å) plotted on the surface of the locally refined cryoEM map of D1D2:RUPA-58:RUPA-154 region. (r) Local resolution (Å) plotted on the surface (left panels) and center (right) of the locally refined cryoEM map of D1D2:RUPA-58:RUPA-154 region further enhanced with EMReady2. All scale bars are 50 nm.

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