Extended Data Fig. 1: Cryo-EM data processing of full-length human dynein-1 in the presence of 5 mM ATP.
From: The mechanochemical cycle of reactive full-length human dynein 1
(a) A representative negative staining micrograph (total 100 micrographs) of purified full-length human dynein-1 and corresponding 2D class averages. (b) A typical cryo-EM micrograph (total 33,302 micrographs) and the flowchart of cryo-EM image processing. (c) Image processing of full-length phi dynein and tail region. (d, e) Orientation distribution of phi dynein and a representative state from open dynein. (f, g) The Fourier shell correlation (FSC) curves of the motor domain in different states and the tail domain. Datasets for dynein in other nucleotide conditions (ATP-Vi, AMPPNP, ADP, apo) were processed similarly.
