Extended Data Fig. 2: TRIM28 shows positive correlation with CtBP.
From: Reciprocal stabilization of CtBP and TRIM28 represses autophagy to promote metastasis
(a) The mRNA levels of CtBP1, CtBP2 and TRIM28 in clinical breast normal (n = 98), and stage I (n = 202), stage II (n = 787), stage III/IV (n = 310) tumor tissues was analyzed based on TCGA-BRCA dataset. (b) The transcriptional Pearson correlation between CtBP and TRIM28 was analyzed based on TCGA-BRCA, CCLE-BRCA and GTEx-Breast datasets. Statistical analysis was performed using a two-tailed, Pearson correlation coefficient test. (c, d) WB of protein level of TRIM28 and CtBP in different types of breast cancer cells (c). The quantification was performed by image J software. The Pearson correlation between TRIM28 and CtBP was plotted after normalized to GAPDH, n = 12 (d). Statistical analysis was performed using a two-tailed, Pearson correlation coefficient test, r = 0.8246, p = 0.0010. (e) PLA assay detecting CtBP and TRIM28 interaction on tissue array including clinical breast normal or cancer samples. PLA signal intensity was quantitated and plotted between normal and different stages of cancer tissues (Normal, n = 25; Stage I&II, n = 129; Stage III&IV, n = 87). (f) Kaplan-Meier survival analysis of overall survival (OS) (TCGA.BRCA.sampleMap/HiSeqV2) based on TRIM28 and CtBP expression in the breast cancer patients from TCGA datasets. Patients were classified as TRIM28highCtBP2high (n = 125) and TRIM28lowCtBP2low (n = 547). Statistical analysis was performed using Log-rank based on Mantel-Cox test, p = 0.0135. Data are shown as the mean with s.d. (a,e); n = 3 biologically independent replicates (c). Statistical analysis was performed using a two-tailed, unpaired Student’s t-test (a,e); n.s. P > 0.05, *P < 0.05, **P < 0.01 and ***P < 0.001.
