Extended Data Fig. 4: Mass spectrometric identification of lysine residues of ArpC4 modified by UFM1. | Nature Structural & Molecular Biology

Extended Data Fig. 4: Mass spectrometric identification of lysine residues of ArpC4 modified by UFM1.

From: Akt-phosphorylated UFL1 UFMylates ArpC4 to promote metastasis

Extended Data Fig. 4

(a–g) Recombinant MBP-ArpC4 and the UFMylation components were subjected to in vitro UFMylation assay. The products were separated by SDS-PAGE, followed by commassie blue staining. The band of UFMylated ArpC4 was cut and delivered to mass spectrometry analysis. Lys35 (a), Lys44 (b), Lys60 (c), Lys77 (d), Lys84 (e), Lys107 (f), and Lys166 (g) of ArpC4 were identified as the potential UFMylation sites.

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