Extended Data Fig. 2: Tree of P-type ATPases and amino acids related to catalysis and structural architecture that are highly conserved across the P-type ATPase family.

a, In the tree, the clades are labeled and colored as per their known or predicted transport substrates. The P-type ATPase subclass is provided in brackets next to the aforestated label. All the major clades with IQtree bootstrap sport of 90% or higher are marked with a filled circle. Branches of special representatives, namely MgtA, MgtB, SERCA and SPCA1 are separately labeled and indicated with bold branches. The tree is based on a multiple sequence alignment of representatives of the major clades of P-type ATPase, which is in figshare. b, Sequence logos showing conservation of amino acid residues involved in ATP hydrolysis and structural architecture conserved among entire family of P-type ATPases (indicated by black dashed boxes in Extended Data Fig. 1). Letters represent amino acid abbreviations and height represents the probability of conservation in the P-type ATPase family. As in Extended Data Fig. 1, gray circles denote residues present at the dimer interface, red circles denote residues involved in ATP hydrolysis, green circles denote residues near Mg²⁺ in the transmembrane domain, and black circles denote residues near Mg²⁺ in the cytoplasmic domain in our dimeric structures, as annotated in Figs. 2–4. Gray, red, green and black arrows, respectively, indicate residues located at the dimer interface, involved in ATP hydrolysis, surrounding the transmembrane or cytoplasmic Mg²⁺, which were mutated in subsequent experiments.