Extended Data Fig. 9: Cryo-EM reconstruction.
From: A scaffold for quinone channeling between membrane and soluble bacterial oxidoreductases
a. ForCE cryo-EM data processing workflow. b. Motion-corrected micrograph of ForCE complex. c. Fourier-shell-correlation plots for independently refined half-maps. Horizontal blue lines indicate Fourier-shell-correlation values of 0.143. d. Examples of 2D classes of the final selection of particles. Number of the particles and estimated resolution in each 2D class are indicated. e. Guinier plot of the final homogeneous refinement indicating a sharpening B-factor of 119.5 Å2. f. Angular distribution of the particles used for the final reconstruction.
