Extended Data Fig. 5: EGFP-trap pull-down of B4GT-HA-EGFP-HT2 from HEK293 cells.
From: Conformational plasticity of a BiP–GRP94 chaperone complex
The Golgi and ER-localized B4GT-HA-EGFP-HT2 fusion protein was pulled down from HEK293 cells. Western blot analysis of input and elution fractions probing for the bait protein, BiP, and GRP94 is shown. HyT36-treatment increased the amounts of BiP and GRP94 pulled down with B4GT-HA-EGFP-HT2, while treatment with the small molecule HALTS1, a HT2-stabilizer, decreased chaperone binding demonstrating that BiP and GRP94 interact with HT2 fusion proteins in cells, depending on the HT2 folding state.
