Extended Data Fig. 6: Characterization of negative-stain EM sample.
From: Conformational plasticity of a BiP–GRP94 chaperone complex
(a) SDS-PAGE gel analysis of GraFix fractions. Gradient density increases with fraction number. Fraction 12 was analyzed by negative-stain EM. Protein bands were detected by Coomassie staining. (b) Western blot analysis of input and gradient fractions 12, 13, and 14 containing glutaraldehyde. The use of different gels accounts for the different banding pattern compared to (a). The asterisk denotes potentially aggregated HT2 protein. (c) Representative negative-stain EM micrograph and 2D class averages obtained from fraction 12. Scale bar represents 200 nm.
