Extended Data Fig. 9: Transcriptional changes upon cellular arsenite exposure.

a, Volcano plots of RNA sequencing data showing the fold-change (Log₂FC) of transcript levels in TXNL1 knockout (KO) HEK-293T cells complemented with wild type (WT) TXNL1, D162R/R234D TXNL1 deficient in proteasome binding, or SxxS TXNL1 deficient in thioreductase activity compared to KO cells without (top) or with (bottom) arsenite treatment. Two-sided Wald test p-values were adjusted for multiple comparisons using the Benjamini and Hochberg method. Note: TXNL1 is the main transcript that is significantly changed in both conditions. b, Volcano plots of RNA sequencing data as in a but showing the fold-change (Log₂FC) of transcript levels in TXNL1 KO cells without (left) or with complementation with WT TXNL1 or the indicated TXNL1 mutants upon arsenite treatment. Note: changes in stress-responsive transcript (purple) levels are not impacted by TXNL1 expression.