Extended Data Fig. 4: MDK or ubiquitin cannot independently assemble into fibrils.
From: Midkine attenuates amyloid-β fibril assembly and plaque formation
a, ThT assay of MDK alone at different concentrations (n = 3 replicates, averaged data shown). b, Time course CD spectra of MDK (0-24 h). c, Negative stain EM of the MDK samples, with a scale bar (100 nm). Similar results were obtained in two independent experiments. d, ThT assay of Ub alone at different concentrations (n = 3 replicates, averaged data shown). e, Time course CD spectra of Ub (0-24 h). f, Negative stain EM of the Ub samples, with a scale bar (100 nm). Similar results were obtained in two independent experiments. Full statistical information is in Source Data Statistics.
