Extended Data Fig. 4: Cryo-EM data processing of the IST2 transmembrane domain in lipid nanodiscs.
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
a, Representative CTF-corrected micrograph for IST2716ND. b, Representative final 2D classes for IST2716ND. c, Data processing workflow for IST2716ND performed in cryoSPARC leading to two distinct reconstructions based on nanodisc size, termed ‘narrow’ and ‘wide’. d, GSFSC curve used for resolution estimation of final map of IST2716ND ‘narrow’ conformation. e, Angular viewing direction distribution of particles contributing to the final map of IST2716ND ‘narrow’. f, Local resolution estimation for IST2716ND ‘narrow’ computed in cryoSPARC. g, GSFSC curve used for resolution estimation of final map of IST2716ND ‘wide’ conformation. h, Angular viewing direction distribution of particles contributing to the final map of IST2716ND ‘wide’. i, Local resolution estimation for IST2716ND ‘wide’ computed in cryoSPARC.
