Extended Data Fig. 5: Features of the IST2 transmembrane domain.
From: Structural basis for lipid transport at membrane contact sites by the IST2–OSH6 complex
a, Superposition of NTDs of IST2 and nhTMEM16. Secondary structure elements of IST2 are labeled. b, Location of the NTD (red) in the IST2 subunit. Ribbon representation of the subunit is shown left. Blowup of the marked region right. c, Dimer interface. Left, IST2 subunit with α10 of the contacted subunit displayed as ribbon. Boxes indicate magnified regions shown right. Top, interacting residues between symmetry related helices α10 and bottom, the α10-α3 interface. Sidechains of interacting residues are shown as sticks. d, Features of the detergent micelle displaying a pronounced distortion at both entrances to the lipid permeation path. The view is towards the subunit cavity from within the membrane. The blurred density corresponding to the protein envelope including the detergent micelle is superimposed on the high-resolution density of the protein (grey) and colored according to its distance to the center of the presumed membrane plane (yellow, distances in nm). The distorted and undistorted widths of the detergent micelle are indicated by dashed and solid lines, respectively. Right panel shows view with the front part clipped. e, Bulky lipid-like density (magenta) surrounding the membrane-inserted region of IST2716 detergent dataset.
