Extended Data Fig. 5: Structures of the core translocase subunits in TIM23 complex.
From: Dynamic TOM–TIM23 supercomplex directs mitochondrial protein translocation and sorting
a, Superimposition of the idle (PDB ID: 8SCX) and active structure of Tim17-Tim23-Tim44. The subunits in the idle structure are all colored grey. Tim17, Tim23, and Tim44 in the active structure are colored the same as in Fig. 2. b, Superimposition of Tim17 in the idle (grey) and active (brownish pink) structures. c, Superimposition of Tim44-CTD in the idle (grey) and active (dark sea green) structures. d, Fitting of the idle Tim23 and Tim44 structures into the density of the active state. The density map is low pass filtered to 6 Å to emphasize the overall fitting the model. Views are shown from the Tim23 side (left) and top (right). Idle Tim23 and Tim44 are colored cyan and magenta, respectively. Tim44 in the active state is colored lime. TM3-4 of the Tim23 idle structure is outside the density map of the active state. The side chains of the Tim44 α2-3 helices in the active state are shown as sticks, with hydrophobic and hydrophilic residues labeled in black and lime, respectively. The shift of the Tim44 α2 and α3 helices is indicated by an arrow. e, Comparison of cryo-EM densities of the active and inactive TIM23 complexes (EMD-27825). Side views illustrate the thinned membrane at the Tim23 side of the active structure. The density map is low-pass filtered to 15 Å, with estimated membrane thicknesses labeled. f, Alignments of Tim17 sequences from representative eukaryotic species. The sequences are from Saccharomyces cerevisiae (S. cerevisiae, Uniprot P39515), Neurospora crassa (N. crassa, Uniprot P59670), Schizosaccharomyces pombe (S. pombe, Uniprot P87130), Kluyveromyces lactis (K. lactis, Uniport Q6CXS9), Candida glabrata (C. glabrata, Uniprot A0A0W0CKS7), Caenorhabditis elegans (C. elegans, Uniprot O44477), Drosophila melanogaster (D. melanogaster, Uniprot Q9VGA2), Mus musculus (M. musculus, Uniprot Q545U2), Homo sapiens (H. sapiens, Uniprot Q99595). g, Alignments of Mgr2 sequences from representative eukaryotic species. The sequences are from Saccharomyces cerevisiae (S. cerevisiae, Uniprot Q02889), Neurospora crassa (N. crassa, Uniprot Q7SDL7), Schizosaccharomyces pombe (S. pombe, Uniprot P79082), Caenorhabditis elegan (C. elegans, Uniprot Q93511), Drosophila melanogaster (D. melanogaster, Uniprot Q9VUM2), Mus musculus (M. musculus, Uniprot P60603), Homo sapiens (H. sapiens, Uniprot P60602).
