Extended Data Fig. 1: Purification and structure determination of the TOM-TIM23 supercomplex.
From: Dynamic TOM–TIM23 supercomplex directs mitochondrial protein translocation and sorting
a, Size exclusion chromatography of the TOM-TIM23 supercomplex. The grey-shaded elution fractions are analyzed by BN-PAGE as shown on the right. b, Representative raw image of the supercomplex particles. c, Representative 2D classes of the supercomplex. Individual components of the supercomplex, including sfGFP, TOM, TIM23, and Fab, are visible in some classes. d, Flow chart of data processing for structure determination of the TOM complex. Details of data processing are provided in the Methods section. e, Focused 3D classification of the substrate in the Tom40 channel. f, Eulerian angle distribution of the particles used for TOM reconstruction, generated from RELION. g, Gold standard Fourier shell correlation (FSC) curve. The estimated resolution of the final map at 0.143 is generated from RELION.
