Extended Data Fig. 2: Structure determination of TIM23 in the supercomplex.
From: Dynamic TOM–TIM23 supercomplex directs mitochondrial protein translocation and sorting
a, Flow chart of data processing for structure determination of the TIM23 complex. The starting particles are derived from the TOM calculation (Extended Data Fig. 1d). b, Representative 2D classes of the TIM23 complex. c, Gold standard Fourier shell correlation (FSC) curve. The estimated resolution of the final map at 0.143 is generated from CryoSPARC. d, Eulerian angle distribution of the particles used for TIM23 reconstruction, generated from CryoSPARC. e, 3D classification of the TOM-TIM23 supercomplex using the selected particles from focused TOM and TIM23 classifications and refinements. f, Distribution landscape of TIM23 relative to TOM. The dots indicate the spatial positions of TIM23 relative to TOM. The dots are color-coded by particle density, with red representing the areas of highest concentration.
