Extended Data Fig. 1: Components purified for reconstituting complexes, and cryo-EM data.
From: Activation of Arp2/3 complex by a SPIN90 dimer in linear actin-filament nucleation
a, Coomassie stained SDS-PAGE gel of proteins used for the structural studies and for pyrene actin polymerization assays. b, A representative micrograph out of a dataset comprising of 24,379 micrographs highlighting the two complexes: Singlets circled in orange and doublets in green, respectively. Scale bar: 100 nm. Different views of the initial 2D class averages of the singlet complex (highlighted in orange) and doublet complex (highlighted in green) are shown on the right. These were used as initial templates for particle picking from micrographs.
