Extended Data Fig. 3: Specific Atg1217BH–Atg17 interaction is important for the condensation at the droplet both in vitro and in vivo.
From: Phase separation promotes Atg8 lipidation and vesicle condensation for autophagy progression
a, Condensation of GFP–Atg1217BH at the early PAS droplets was impaired by mutations. Scale bar = 10 μm. b, Cells expressing Atg12 or Atg17 mutants were treated with rapamycin, and the amounts of Atg12-Atg5-GFP and Atg17-2×mCherry were analyzed by immunoblotting using anti-GFP and anti-mCherry antibodies. c, PAS targeting of the Atg12–Atg5–Atg16 complex was impaired by mutations. Scale bar = 5 μm. d, Overall view of Fig. 3e. The box represents the interquartile range (IQR) with the median indicated by a horizontal line; whiskers extend to the most extreme data points within 1.5×IQR from the quartiles, and points beyond are plotted as outliers. Number of PAS puncta: n = 1,548 (WT), 1,990 (V43A), 1,815 (L47A), 1,710 (L54A), and 1,829 (L57A).
