Extended Data Fig. 1: Purification of recombinant proteins and characterization of tagging effects on autophagy.
From: Phase separation promotes Atg8 lipidation and vesicle condensation for autophagy progression
a, SDS-PAGE image of the proteins used for Fig. 1 experiments. b, Cells expressing Pgk1-GFP were treated with rapamycin and examined by immunoblotting using an antibody against GFP. The band intensities of Pgk1-GFP and its cleaved fragments (GFP’) were measured using Fiji. The proportion (%) of GFP’ relative to the total signal (Pgk1-GFP + GFP’) was calculated and regarded as the degradation of Pgk1-GFP. Bars represent means ± s.d., n = 3 biological replicates. (a two-sided Tukey’s multiple comparisons test with adjustments for multiple comparisons). c, FRAP analysis of the Atg12-Atg5-Atg16 complex at Early PAS droplets. Bars represent means ± s.d., n = 3 biological replicates. Scale bar = 2 μm.
