Extended Data Fig. 2: NMR analysis of GB1–Atg1217BH.
From: Phase separation promotes Atg8 lipidation and vesicle condensation for autophagy progression
a, Main chain assignment results are shown on [1H-15N] heteronuclear single quantum coherence spectroscopy (HSQC); GB1-derived signals are labeled in black, and Atg12-derived signals are labeled in blue. b, Superposition of [1H-15N] HSQC obtained by titration experiments. c, Signal intensity ratios before and after the addition of Atg17–Atg31C are plotted by residue (GB1 is shown in gray and Atg12 in orange, 0.1, 0.5, and 1.0 molar equivalents from top to bottom). The signal-to-noise ratio of the signals was used to calculate the error bars (n = 1 experiment). d, Secondary structure propensity analysis results (GB1 is shown in gray and Atg12 in orange). The secondary structure position in the GB1 structure is attached.
