Extended Data Fig. 8: TORC1 amino acid signalling in SEACIT deletion strains.
From: Structure and function of the yeast amino acid-sensing SEAC–EGOC supercomplex
a, Immunoblot of phosphorylated and total Sch9 after starvation and repletion of amino acids in wild type cells. b, Immunoblot of phosphorylated and total Sch9 after starvation and repletion of amino acids in Δsea1, Δnpr2 and Δnpr3 cells. c, Immunoblot of phosphorylated and total Sch9 after starvation and repletion of amino acids in Npr2R84AN85A cells. d, Quantification of relative Sch9 phosphorylation over 30 minutes of starvation and amino acid repletion in wild type and SEACIT deletion strains. Data from three independent experiments, except for Δnpr3 (two independent experiments), are presented as mean values +/- SD. e, Quantification of relative Sch9 phosphorylation over 30 minutes of starvation and amino acid repletion in wild type, Δsea1 and Npr2R84AN85A cells. Data from three independent experiments are presented as mean values +/- SD.
