Supplementary Figure 5: Number of quantified proteins and phosphorylation sites across three laboratories. | Nature Protocols

Supplementary Figure 5: Number of quantified proteins and phosphorylation sites across three laboratories.

From: Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry

Supplementary Figure 5

The total number of quantified A) proteins and B) phosphorylation sites that could be assigned to human are illustrated as ‘UpSet’ plots (Lex, 2014). Horizontal bars indicate total number of proteins or phosphorylation sites detected by each laboratory; vertical bars depict the number of jointly detected features, as indicated by the layout matrix below. C) Comparison of detected and quantified human phosphosites in two TMT10-plexes acquired in PCC1. D) and E) Analogous plots for PCC2 and PCC3, respectively. PDX models used in this study were approved by the institutional animal care and use committee at Washington University in St. Louis

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