Supplementary Figure 3: Examples of NGS analysis of three individual F0 Tyrosinase (Tyr) CRISPR axolotls. | Nature Protocols

Supplementary Figure 3: Examples of NGS analysis of three individual F0 Tyrosinase (Tyr) CRISPR axolotls.

From: Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)

Supplementary Figure 3

(a) An animal harbouring only two types of modifications (homozygous-type) at the Tyr-gRNA targeted locus. It likely represents modifications which occurred separately on the paternal and maternal alleles in a single cell stage axolotl eggs. (b) A mosaic animal harbouring 100% modified Tyrosinase locus, with many types of indels. (c) An animal with incompletely modified Tyrosinse locus; a proportion (approximately 16%) of the alleles are unmodified. Tyr-gRNA target (red); PAM sequence (blue); characters in squares indicate single nucleotide substitutions (SNS), which appear only at very low percentage and likely represent mutations generated during PCR. Therefore, we categorize the same type of modification with or without SNS as one group.

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