Supplementary Figure 2: Examples of NGS PCR on individual F0 CRISPR axolotls injected with gRNA-CAS9-NLS RNP complexes. | Nature Protocols

Supplementary Figure 2: Examples of NGS PCR on individual F0 CRISPR axolotls injected with gRNA-CAS9-NLS RNP complexes.

From: Application and optimization of CRISPR–Cas9-mediated genome engineering in axolotl (Ambystoma mexicanum)

Supplementary Figure 2

Examples of NGS-PCR1 (a) and NGS-PCR2 (b) on individual F0 CRISPR axolotls from two independent genomic loci:, Tyrosinase and Yap1. Red stars indicate the PCR products carrying large indels, which run faster on the gel than the PCR products amplified from unmodified alleles or those carrying small indels. All products from NGS-PCR2 should be sequenced, since it is impossible to determine the genotype solely by the gel picture (PCR products harbour small indels are indistinguishable from the wild-type PCR product).

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