Supplementary Figure 1: Gating strategy. | Nature Protocols

Supplementary Figure 1: Gating strategy.

From: Monitoring early S-phase origin firing and replication fork movement by sequencing nascent DNA from synchronized cells

Supplementary Figure 1

Typical flow cytometry profiles of U2OS cells (with normal levels of cyclin E) that were released into medium containing EdU (25 µM) for 14 h after mitosis exit. The strategy used to analyse the flow cytometry profiles consists of first gating the cells based on their distribution in the forward scatter/ side scatter plot (gate A, left panel); then, a second gate is applied to eliminate cell doublets on the propidium iodide (PI) peak height versus integral plot (gate B, center-left panel). After gating, the DNA content histograms and the EdU versus DNA content density scatter plots of the cells are plotted.

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