Supplementary Figure 7: Key histological characteristics of UM-UC-13 and T24 xenograft models.
From: A surgical orthotopic approach for studying the invasive progression of human bladder cancer
The xenografted tumors display less aggressive pathobiological features and require a higher inoculum for their development. The figure shows representative histological results after implantation of 5 ×106 UM-UC-13 and 1.5 ×106 T24 human bladder tumor cells into the bladder lumen of NOD-SCID mice. The tumors developed for four weeks until end point. a, Characteristic histological analysis at the invasive edge of the tumor. Cytokeratin 8+ (CK8, clone EP17, Epitomics, 1:100, cat. no. AC-0007) positive cancer cells are observed invading the muscle of the bladder. In general, the mice exhibit muscle-invasive T2 bladder tumors at a solitary focal location. b, Lymphovascular invasion is also observed at lower frequency. CK8 positive tumor cells are detected within CD31 (Dianova, clone SZ31, 1:50, cat. no. DIA-310) and LYVE1 (Abcam, 1:1000, cat. no. ab14917) positive vessels. Scale bars, 50 μm.
