Supplementary Figure 1: Statistics for 1 million cells analyzed by MARS-seq2.0. | Nature Protocols

Supplementary Figure 1: Statistics for 1 million cells analyzed by MARS-seq2.0.

From: MARS-seq2.0: an experimental and analytical pipeline for indexed sorting combined with single-cell RNA sequencing

Supplementary Figure 1

UMI and gene counts distributions on 1,033,944 mouse cells using genome assembly mm9 and 16,488 empty wells analyzed by MARS-seq2.0. Cells analyzed were either immune, epithelial, fibroblast, stromal, hepatocytes or cancer mouse cells as well as cell lines (mouse ES and MEF). (A-B) A histogram showing the fraction of cells (black) and empty wells (red) binned by logarithmic intervals of UMI-counts (A) or by gene-counts (B). (C) Empty wells are enriched with lowly detected genes. Histogram showing the fraction of cells/wells (similar to A-B) binned by the average number of UMIs observed per gene (log2 scale).

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