Supplementary Figure 1: Example of sequin calibration.
From: Use of synthetic DNA spike-in controls (sequins) for human genome sequencing
Genome browser views show sequencing alignments within a single sequin standard before (upper) and after (middle) coverage calibration, performed using anaquin ‘calibrate’. During calibration, sequin alignments are down-sampled to achieved matched coverage with the human sample DNA (lower) within sequin regions. This example also shows artifactual enrichment of read-pairs at sequin termini, which occurs during some library preparation methods. Anaquin ‘calibrate’ automatically removes these terminal alignments before calibration. Sequin edge regions (550 bp, by default) are also excluded during the calibration process, as well as downstream anaquin analyses (germline/somatic).
