Supplementary Fig. 1: Orthogonal stimulation of red- and blue-sensitive optogenetic probes requires simultaneous control over three wavelengths of light.
From: High-throughput multicolor optogenetics in microwell plates
a) PhyB/PIF and iLid/sspB are red- and blue-light responsive heterodimers, respectively. b) While iLid does not absorb light beyond 500 nm, PhyB absorbs light across the visible spectrum, challenging orthogonal multiplexing. Lines above absorption spectra indicate the wavelengths used for activation. Spectra are adapted from prior literature2,3. c) Far-red light can be applied concurrently with blue light to avoid PhyB cross-activation.
