Supplementary Fig. 3: Linear control of illumination intensity. | Nature Protocols

Supplementary Fig. 3: Linear control of illumination intensity.

From: High-throughput multicolor optogenetics in microwell plates

Supplementary Fig. 3

Programmed light intensity settings are linearly correlated to measured light output. Blue light power density from a 1-color (blue) optoPlate-96 was measured. Data represent means of three readings from 2 independent wells per condition. Error bars indicate range. Power was measured using a Thorlabs PM20A power meter coupled to a multimode optical fiber (Thorlabs M71L01). Light was collected by placing the fiber at the bottom of a 96-wellplate well that was sitting atop the optoPlate adaptor. Due to inefficiencies in light capture and losses in light transmission to the power meter, the power densities presented here underestimate the blue light power density at the sample.

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