Supplementary Fig. 6: MTSL reduction in the E. coli cell suspension. | Nature Protocols

Supplementary Fig. 6: MTSL reduction in the E. coli cell suspension.

From: In situ observation of conformational dynamics and protein ligand–substrate interactions in outer-membrane proteins with DEER/PELDOR spectroscopy

Supplementary Fig. 6

Cells (OD600 = ~80) were incubated with 500 μM MTSL (indicated by the dashed line) in the spin labeling buffer. Samples were periodically collected at the indicated time points, pelleted, and the MTSL concentration in the supernatant was monitored using RT CW EPR spectroscopy. For the zero-time sample, cells were pelleted immediately after mixing with MTSL (overall, which took an additional 6-7 min including centrifugation and EPR measurement). Error bars indicate the 15% error, which is typical for spin quantification using RT CW EPR spectroscopy. Similar trends were observed in independent experiments.

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