Supplementary Fig. 1: Purification of Ssb-bound ribosomes depends on the presence of nascent chains.
From: Selective ribosome profiling to study interactions of translating ribosomes in yeast
(a) Western blot analysis of three Ssb1-GFP purifications performed under low salt (LS: 140 mM KCL) or high salt conditions (HS: 500 mM KCL) and in presence of cycloheximide (CHX) or puromycin (Puro). The upper panel shows a western blot developed using Ssb antibodies whereas the lower western blot was developed with antibodies detecting ribosomal protein Rpl35. (L: lysate, P: resuspended ribosomes used as input for AP, U: unbound, supernatant of AP, W: first wash fraction, B: bound fraction of AP.) (b) Bioanalyzer results of a Nano chip to measure the co-purified RNA in the bound fractions of the APs. The Figure was published previously as FigureS2 (c) in11.
