Supplementary Fig. 5: Multi-antibody qualification of CD11b antibodies by t-CyCIF in human FFPE tonsil tissue.
From: Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging
Individual and merged a) low (Scale bars: 100 µm) and b) high (Scale bars: 50 µm) magnification images of immunofluorescence from three CD11b antibodies: EP1345Y, Abcam; C67F154, eBioscience; and EPR1344, Abcam. c) Plots of the pixel-by-pixel correlation of the signal intensity generated by the CD11b antibodies. The plots display the correlation of a random sampling of 2,000 pixels. The plots on the lower left (with blue dots) show the original fluorescence intensity at each pixel, and the plots on the upper right (with cyan dots) show the log-transformed fluorescence intensity at each pixel. The Pearson correlation coefficients (R) are shown. DR, dynamic range. The dynamic range and correlation values from 5 different regions are presented in Supplementary Table 1.
