Supplementary Fig. 14: Geographic visualization of t-CyCIF data in LUNG-1-LN and LUNG-2-BR.
From: Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging
a) Montage of t-CyCIF images and b) corresponding dot plot for tumour cells (keratin+, blue), fibroblasts (α-SMA+, green) and immune cells (CD45+ or IBA1+, red) in LUNG-1-LN. c) Representative merged images of t-CyCIF data for α-SMA, keratin, CD45RB, IBA1, CD20, and CD3 from LUNG-1-LN (Scale bars: 50 µm). d) Montage of t-CyCIF images and e) corresponding dot plot for tumour cells (keratin+, blue), fibroblasts (α-SMA+, green) and immune cells (CD45+ or IBA1+, red) in LUNG-2-BR. f) Representative merged images of t-CyCIF data for α-SMA, keratin, CD45RB, IBA1, CD20, and CD3 from LUNG-2-BR (Scale bars: 50 µm).
