Supplementary Fig. 19: Galleries of images of immune markers expressed in rare cell clusters 5, 6, and 7.

Galleries of images of immune markers (LAG3, PD-1, CD45RB, CD3, PD-L1, CD4, CD45, CD8a, CD163, CD68, CD14, CD11b, FOXP3, IBA1, CD20) and DNA stain for 10 individual rare cells in cluster 5 identified using automated methods that putatively express CD45⁺/CD45RB⁺/CD3⁺/CD4⁺/CD20⁺/PD-1⁺ (top), 6 individual rare cells in cluster 6 that putatively express CD45⁺/CD45RB⁺/IBA1⁺/CD163⁺/CD14⁺/CD68⁺/CD11b⁺/PD-1⁺/LAG3⁺/PD-L1⁺ (middle), or 6 individual rare cells in cluster 7 that putatively express CD45⁺/CD45RB⁺/CD3⁺/PD-1⁺ (Scale bar: 25 µm). Supplementary Table 10 indicates whether visual review of these images by a trained pathologist was deemed to be consistent with the automated calls.