Supplementary Fig. 1: Schematic of setup used for shielded virus stamping for experiments.
From: Magnetically guided virus stamping for the targeted infection of single cells or groups of cells
To maintain health of cells during the stamping procedure and allowing for glass capillary (pipette), optical and magnet access we built a custom chamber made of two concentric compartments31. The inner compartment is a glass-bottomed dish in which cells are cultured at 37 °C. The surrounding compartment is continuously filled via an inlet with humidified (<98% relative humidity) synthetic air (80% N2 and 20% O2) supplemented with 5% CO2. The top of the chamber is left open to access the cells by the glass capillary pipette and objective (top-down dipping objective). A 35 mm diameter opening of the outer chamber (chamber lid) allows optical access from the top. The angle between pipette and glass-bottom dish, which is usually ~ 45° can be adjusted according to the experimental needs. The bottom of the chamber can be optically assessed as well as indicated by the condenser. Depending on the angle of the pipette, the electromagnet (or permanent magnet) is aligned from below without blocking the light pathway. If experiments are to be conducted over extended time periods, the chambers can be covered with silicone sealant to enable gas exchange between compartments and to prevent evaporation of the cell-culture media.
