Supplementary Fig. 3: Result of autofocus simulation for MOVIE performance.
From: Advanced CUBIC tissue clearing for whole-organ cell profiling
MOVIE-focus was simulated by 400,000 images data sets (400 of different light-sheet positions with 0.35 μm span) which was acquired by our LSFM at the 5 μm step size covering 5000 μm depth, using a PI stained mouse brain. MOVIE (−) used the constant light-sheet position throughout. MOVIE (+) used parameters of Supplementary Fig. 2a. Scale bar, 100 μm.
