Supplementary Fig. 2: Temporal pattern of transgenic microRNA expression in vivo. | Nature Protocols

Supplementary Fig. 2: Temporal pattern of transgenic microRNA expression in vivo.

From: Engineering, delivery, and biological validation of artificial microRNA clusters for gene therapy applications

Supplementary Fig. 2

Real Time RT-PCR quantification of mature microRNA expression in human G30 GBMs recovered from mice brains at the times labelled in the X axis. Day 7 and Day 19 refers to number of days after the intracranial infection with the AAV vectors. Control refers to ex-vivo tumors infected with control AAV expressing scrambled microRNA transgene and analyzed at Day 7 after infection. Represented are Mean ± SD from n=3 independent experiments. *** = p<0.001 (Student’s t-test, 2 tails).

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