Supplementary Figure 2: Protocol optimization.
From: Using TTchem-seq for profiling nascent transcription and measuring transcript elongation
(a) Increasing treatment time of total RNA with sodium hydroxide (0.167 M) results in decreasing RNA fragment size. 1.2% denaturing TBE gel. (b) Bioanalyzer results comparing Qiagen minElute RNA clean-up of 4SU-RNA after streptavidin pull-out using the recommended protocol which selects for RNA > 200 nt or the modified protocol using an increased volume of ethanol.
