Supplementary Figure 2: Fluorescence minus one (FMO) controls and FACS results of incomplete liver dissociation. | Nature Protocols

Supplementary Figure 2: Fluorescence minus one (FMO) controls and FACS results of incomplete liver dissociation.

From: Isolation of tissue-resident vascular endothelial stem cells from mouse liver

Supplementary Figure 2

(a) Flow cytometric analysis of the liver showing the distribution of fluorescence of the FMO control and unstained control. (b) Flow cytometric analysis of the liver comparing successful (left) and incomplete (right) dissociation resulting in different percentages of cells in the CD31+CD45−fraction. All animal experiments in this figure were performed in accordance with the Institutional Animal Care and Use Committee of Osaka University and received approval from the Institutional Review Board.

Back to article page