Supplementary Fig. 2: The three step sequential gating strategy for the isolation of mouse embryo cells.
From: GOTI, a method to identify genome-wide off-target effects of genome editing in mouse embryos
a, Size fractionation on the basis of forward scatter Height (FSC-Height) and side scatter Height (SSC-Height). b, Elimination of cell debris or aggregates on the basis of SSC height and area. c, Selection of tdTomato+ Cells and tdTomato− cells. The FSC/SSC gates of the starting cell population were set to include all cells. Then, doublet cells were excluded by SSC-H versus SSC-A. Positive and negative boundaries were defined by control progeny cells of non-edited blastomeres.
