Extended Data Fig. 4: FISH in combination with antibody staining of hepatic duct cells in the liver and secretory cells in the intestine at 20 dpf. | Nature Protocols

Extended Data Fig. 4: FISH in combination with antibody staining of hepatic duct cells in the liver and secretory cells in the intestine at 20 dpf.

From: Combined whole-mount fluorescence in situ hybridization and antibody staining in zebrafish embryos and larvae

Extended Data Fig. 4

a–d, Labelling of FISH-anxa4 (the antigen of 2F11), 2F11 (the marker of hepatic duct cells and secretory cells in the intestine) and DAPI in hepatic duct cells in the liver and secretory cells in the intestine in wild type at 20 dpf (3D stack of images; 337.33 µm, 38 slices, 8.877 µm each slice; left view). BF, bright field. Scale bar, 100 µm.

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