Fig. 3: Screenshots showing the use of Excel macros to calculate TCID50 (Supplementary Method 1 and Box 1) for titration assay and titers for neutralization assay (Supplementary Method 2 and Box 1).
From: Quantification of SARS-CoV-2 neutralizing antibody by a pseudotyped virus-based assay
a, Luminescence readout data for a titration assay exported in the form of an Excel file. The selected raw data are indicated in a rectangle. b, The macro file for titration of the pseudotyped virus, TCID50_SARS-CoV-2 (Supplementary Method 1). A rectangle indicates where the raw data should be pasted. c, Calculated results in the TCID50_SARS-CoV-2 macro (Supplementary Method 1). d, Luminescence readout data for a neutralization assay exported in the form of an Excel file. The selected raw data are indicated in a rectangle. e, The macro file for the neutralization assay, Neutralization_SARS-CoV-2 (Supplementary Method 2). A rectangle indicates where the raw data should be pasted. f, Calculated results in the Neutralization_SARS-CoV-2 macro (Supplementary Method 2). Dil., dilution; EC50, half maximal effective concentration; P/N, average of virus control/average of cell control; *PsV, pseudotyped virus; **PBNA, pseudotyped virus–based neutralization assay; RLU, relative light unit; TCID50, 50% tissue culture infectious dose; TRF, time-resolved fluorescence.
