Fig. 2: Gel extraction of SARS-CoV-2 fragments, full-length cDNA and full-length viral RNA. | Nature Protocols

Fig. 2: Gel extraction of SARS-CoV-2 fragments, full-length cDNA and full-length viral RNA.

From: Engineering SARS-CoV-2 using a reverse genetic system

Fig. 2

a, Agarose gel showing each SARS-CoV-2 DNA fragment (1µL of each) isolated post–restriction enzyme digestion and utilized for in vitro ligation. b,c, Representative gels from successful (b) and unsuccessful (c) attempts to generate full-length SARS-CoV-2 cDNA. d,e, Representative agarose gels from successful (d) and unsuccessful (e) attempts to generate in vitro transcribed full-length SARS-CoV-2 viral RNA prior to electroporation and virus recovery.

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