Table 1 Restriction enzymes for validation of seven SARS-CoV-2 plasmids
Plasmid | Restriction enzyme(s) | DNA fragments with expected size |
|---|---|---|
pUC57-CoV-2-F1 | BsaI | 3,644 bp (F1)a + 1,367 bp + 1,366 bp |
pCC1-CoV-2-F2 | BsaI | 6,466 bp + 3,886 bp (F2)a + 1,344 bp |
pCC1-CoV-2-F3 | BsaI | 6,466 bp + 4,480 bp (F3)a + 1,344 bp |
pUC57-CoV-2-F4 | BsaI | 5,607 bp (F4)a + 1,367 bp + 1,366 bp |
pUC57-CoV-2-F5 | Esp3I and PvuI | 4,457 bp (F5)a + 1,674 bp + 620 bp + 234 bp + 161 bp+ 42 bp |
pUC57-CoV-2-F6 | Esp3I and PvuI | 4,284 bp (F6)a + 1,674 bp + 620 bp + 234 bp + 161 bp+ 42 bp |
pCC1-CoV-2-F7 | Esp3I and SnaBI | 3,563 bp (F7)a + 2,522 bp + 2,395 bp + 1,689 bp + 651 bp + 553 bp |
