Fig. 1: General overview of the MNA described in Procedure 2. | Nature Protocols

Fig. 1: General overview of the MNA described in Procedure 2.

From: Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays

Fig. 1

Serum samples are diluted in a 96-well plate (Stage I), before wild-type SARS-CoV-2 virus is added to the diluted serum at BSL3 and neutralization allowed to occur (Stage II). The neutralized virus is then transferred onto Vero E6 cells (Stage III), allowed to adsorb, overlaid with viscous medium and incubated for 24 h (Stage IV). Plates are then fixed (Stage V), virus infected foci immunostained (Stage VI) and foci counted (Stage VII). Well counts are then analyzed with SoftMax Pro to determine median neutralization titers of the reference and test sera (Stage VIII).

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