Fig. 3: Protocol for calculating the ND50 for serum by PRNT assay using Probit analysis in R. | Nature Protocols

Fig. 3: Protocol for calculating the ND50 for serum by PRNT assay using Probit analysis in R.

From: Quantification of SARS-CoV-2 neutralizing antibody by wild-type plaque reduction neutralization, microneutralization and pseudotyped virus neutralization assays

Fig. 3

a, Plaque counts should be limited to the average of the VOC counts. b, Counts should be arranged in a new worksheet, as shown, and saved as a .csv file. c, Following analysis using the R script supplied, a graph is generated and automatically saved as an .EMF file. d, An example of a good result with a full curve and tight confidence intervals. e, An example of a graph generated for a nonneutralizing sample with a poor curve.

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